IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to
NPHL@unmc.edu or call (402) 559-9444.
NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see
NUlirt Guide).
Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing
will not be performed without all required information.
Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.
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| Test: |
Mycobacterium tuberculosis Direct Susceptibility Test
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| Synonym: |
MTB DST, Pyrosequencing, PSQ, TB, Mycobacterium tuberculosis, MTBC, first line, second line |
| Method: |
- See California Department of Public Health (CDPH) Microbial Diseases Lab (MDL) Test Directory for Methodology, reflex testing, specimen types, collection/stability, labeling, volume, rejection criteria, interferences/limitations, and turn-around times.
- Mycobacterium tuberculosis Complex: Drug Susceptibility Testing, First-Line
- Mycobacterium tuberculosis Complex: Drug Susceptibility Testing, Second-Line
- Mycobacterium tuberculosis Complex: MDDR by Pyrosequencing Assay
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| Availability: |
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| Specimen: |
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| Collection Device: |
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| Volume: |
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| Storage/Transport: |
- For shipping of isolates, see Category A instructions
- https://www.nphl.org/index.cfm/specimen-packaging-shipping/
- Seal tubes with Parafilm™ or tape and place in biohazard bag with absorbent material
- NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens
- Submit all specimens for MTB DST through the state health departments, NOT directly to CDPH.
- Ship to NPHL on Monday through Thursday to guarantee arrival at CDPH by Friday
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| Unacceptable: |
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| Reference Interval: |
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| Comments: |
- Isolates that are tested at the Microbial Diseases Laboratory will be forwarded to the CDC if further characterization is needed or requested
- Test performed at Microbial Diseases Laboratory, California Department of Public Health, 850 Marina Bay Parkway, Richmond, CA 94804
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| Revised: |
3/5/2024 |
If you have questions about proper specimen collection, please call
NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.
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