IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to
NPHL@unmc.edu or call (402) 559-9444.
NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see
NUlirt Guide).
Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing
will not be performed without all required information.
Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.
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| Test: |
Chikungunya Virus RNA Detection
|
| Synonym: |
Arbovirus, Chikungunya virus RT-PCR
|
| Method: |
RT-Polymerase Chain Reaction (PCR)
|
| Availability: |
https://arupconsult.com/content/mosquito-borne-arboviruses#toc-arup-laboratory-tests
|
| Specimen: |
Serum and cerebrospinal fluid (CSF)
|
| Collection Device: |
https://arupconsult.com/content/mosquito-borne-arboviruses#toc-arup-laboratory-tests
Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms
|
| Volume: |
https://arupconsult.com/content/mosquito-borne-arboviruses#toc-arup-laboratory-tests
|
| Storage/Transport: |
Prior to and during shipment: Frozen on Dry Ice.
Shipping instructions, including specimen-handling requirements during transport: Seal screw-top tubes with Parafilm™ or tape and place in biohazard bag with absorbent material.
Ship in insulated box on dry ice using UN1845 label. Place packing list or other paperwork on top of Styrofoam and lid. Mark out rigid box as UN3373 Category B, Biological Substance. Transport by NPHL ground courier. If courier not available, ship by FedEx, UPS or USPS (1st Class, Priority or Express Mail). Follow appropriate DOT/IATA Regulation Guidelines. For assistance, call NPHL State Training Coordinator at (402) 559-3590.
See instructions and shipping address: http://nphl.org/
NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.
|
| Unacceptable: |
Severely lipemic, icteric or hemolyzed samples; heat-inactivated serum; multiple freeze thaw cycles. Heparinized specimens.
|
| Specimen Stability: |
2-8°C for 72 h, if > 3 d, freeze
|
| Reference Interval: |
NEGATIVE - No significant level of detectable chikungunya virus RNA
POSITIVE – Chikungunya virus RNA detected
|
| Reportable Disease: |
A positive result is reportable as Arbovirus infection under Title 173, report within 7 days
|
| Comments: |
Pre-approval needed from the state or local health department. Send approval to NPHL@unmc.edu or call (402) 559-9444.
Test Request Order Form must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history.
|
| Revised: |
3/15/2021 |
If you have questions about proper specimen collection, please call
NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.
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