Testing Results

Measles Virus PCR or Genotyping (CDCSO)

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test:	Measles Virus RT-PCR or Genotyping Pre-approval needed from state or local health department Send approval to NPHL@unmc.edu or call (402) 559-9444 NUlirt order: CDCSO Supplemental form needed: Vaccine Preventable Disease (VPD)
Synonym:	Measles detection Measles molecular Rubeola VPD Vaccine preventable disease Measles vaccine Measles
Method:	RT-PCR
Availability:	Test performed Monday - Friday Turnaround time 2 days for PCR, 10 days for genotyping Submit all specimens through the state health departments, NOT directly to the Minnesota Department of Health
Specimen:	Throat swab Buccal swab Nasopharyngeal aspirate or swab Urine
Collection Device:	Store swabs/aspirates in 2 mL viral transport media Seal with Parafilm™ or tape Test subject to CLIA regulations and requires 2 patient identifiers on container and requisition forms
Volume:	250 µL
Storage/Transport:	For shipping, see Category B instructions https://www.nphl.org/index.cfm/specimen-packaging-shipping/ Seal tubes with Parafilm or tape and place in biohazard bag with absorbent material NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens
Unacceptable:	Samples stored incorrectly
Specimen Stability:	Stable at 4 °C for 7 days Store frozen after 7 days
Reference Interval:
Reportable Disease:
Comments:	Genotyping will be perormed on all PCR positive specimens unless otherwise indicated as a part of a larger outbreak Measles vaccine-specific PCR available as appropriate Test performed at the Minnesota Department of Health, Public Health Laboratory, St. Paul, MN Submit all specimens through the NPHL, NOT directly to the Minnesota Department of Health
Revised:	4/15/2025

If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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