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Yersinia spp. (not pestis) (NPHLBK)(ORGISO)  

 
NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test: Yersinia spp. (not pestis)
  • No pre-approval needed
  • NUlirt order for isolates: NPHLBK
  • NUlirt order for stools: ORGISO
 
Synonym:
  • Yersinia enterocolitica, Yersiniosis, Yersinia not pestis, Yersinia species
 
Method:
  • Culture
  • Banking
  • Whole Genome Sequencing
 
Availability:
  • Monday - Friday
  • Turnaround time 1-7 days
 
Specimen:
  • Pure isolate
  • Stool in appropriate media
 
Collection Device:
  • Stool specimen should be placed in Cary Blair or Enteric Plus transport media according to manufacturers instructions
    • Fresh stool should be placed in media within 2 hours of collection
    • Ration of stool/Enteric Plus is 2:15 (0.4mL stoll to 3.75 mL of Enteric Plus)
  • Isolate should be on plate or slant
  • Seal with Parafilm™ or tape
  • Test subject to CLIA regulations and requires 2 patient identifiers and specimen source on container.
 
Volume: Stool sample minimum 1 mL  
Storage/Transport:  
Unacceptable:
  • Leaking specimens
  • Mixed isolates
  • Stool contaminated with urine
 
Specimen Stability:
  • Stool stable refrigerated 2-8°C up to 4 days
  • Isolates table at ambient air 15-25 °C
 
Reference Interval:  
Reportable Disease:  
Comments:
  • Stool culture performed at Nebraska Medicine
  • Banking and Whole Genome sequencing performed at NPHL
  • Test results are to be used for epidemiological and infection control purposes only
 
Revised: 4/29/2024 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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