IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to
NPHL@unmc.edu or call (402) 559-9444.
NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see
NUlirt Guide).
Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing
will not be performed without all required information.
Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.
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| Test: |
Yersinia spp. (not pestis)
- No pre-approval needed
- NUlirt order for isolates: NPHLBK
- NUlirt order for stools: ORGISO
|
| Synonym: |
- Yersinia enterocolitica, Yersiniosis, Yersinia not pestis, Yersinia species
|
| Method: |
- Culture
- Banking
- Whole Genome Sequencing
|
| Availability: |
- Monday - Friday
- Turnaround time 1-7 days
|
| Specimen: |
- Pure isolate
- Stool in appropriate media
|
| Collection Device: |
- Stool specimen should be placed in Cary Blair or Enteric Plus transport media according to manufacturers instructions
- Fresh stool should be placed in media within 2 hours of collection
- Ration of stool/Enteric Plus is 2:15 (0.4mL stoll to 3.75 mL of Enteric Plus)
- Isolate should be on plate or slant
- Seal with Parafilm™ or tape
- Test subject to CLIA regulations and requires 2 patient identifiers and specimen source on container.
|
| Volume: |
Stool sample minimum 1 mL |
| Storage/Transport: |
|
| Unacceptable: |
- Leaking specimens
- Mixed isolates
- Stool contaminated with urine
|
| Specimen Stability: |
- Stool stable refrigerated 2-8°C up to 4 days
- Isolates table at ambient air 15-25 °C
|
| Reference Interval: |
|
| Reportable Disease: |
|
| Comments: |
- Stool culture performed at Nebraska Medicine
- Banking and Whole Genome sequencing performed at NPHL
- Test results are to be used for epidemiological and infection control purposes only
|
| Revised: |
4/29/2024 |
If you have questions about proper specimen collection, please call
NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.
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