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Sars-CoV-2, Influenza, and RSV Multiplex Assay RT-PCR (NCOV4)  

 
NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test: Sars-CoV-2, Influenza, and RSV Multiplex Assay RT-PCR
  • No pre-approval needed
  • NUlirt Order: NCOV4
 
Synonym:
  • COVID
  • Novel coronavirus
  • RSV
  • Respiratory Syncytial virus
  • Influenza A
  • Flu A
  • Influenza B
  • Flu B
 
Method: Qualitative nucleic acid amplified real time RT-PCR assay  
Availability:
  • Test performed Monday - Friday
  • Turnaround time 24-48h
 
Specimen:
  • Nasopharyngeal swab
  • Nasal swab
 
Collection Device:
  • Viral Transport Media  (VTM, UTM, VCM or M4)
  • Specimens should be collected only on mini-tipped swabs with a synthetic tip (such as polyester or Dacron®) with aluminum or plastic shafts
  • Do not use calcium alginate swabs or swabs with wooden shafts, as they may contain substances that inactivate some viruses and inhibit PCR testing
  • Seal with Parafilm™ or tape
  • Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms
 
Volume: 1-3 mL viral transport media  
Storage/Transport:  
Unacceptable:
  • Specimens not kept at 2-8°C (≤7 days) or frozen at -20°C or below
  • Inapproprate specimen type
  • Insufficient specimev volume
  • Swabs submitted with calcium alginate or cotton tips with wooden shafts are not acceptable
 
Specimen Stability: Stable at Refrigerated 2-8°C up to 7 days; Frozen -20°C  >7 days, stable frozen for 30 days    
Reference Interval:  
Reportable Disease:  
Comments:  
Revised: 10/30/2024 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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