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Proof of Cure, Shiga toxin-producing Escherichia coli (SHIGA)  

 
NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test: Proof of Cure, Shiga toxin-producing Escherichia coli (SHIGA)
  • Pre-approval needed from state or local health department
  • Send approval to NPHL@unmc.edu or call (402) 559-9444
  • NUlirt Order: SHIGA
   
Synonym:
  • Proof of cure, Shigatoxin testing, E. coli 0157:H7, Non O157
 
Method:
  • Qualitative Enzyme Immunoassay
 
Availability:
  • Daily
 
Specimen:
  • Stool
  • Pure Isolate
 
Collection Device:
  • Stool specimens should be collected in Cary Blair or Enteric Plus transport meida according to manufacturer's instructions
    • If fresh stool is received, it should be placed into transport media within 2 hours of collection
    • Ratio of stool to Enteric Plus is 2:15 (0.5 mL stool to 3.75 mL Enteric Plus liquid media)
  • Isolates should be on a plate or slant
  • Seal screw-top tubes with Parafilm™ or tape and place in biohazard bag with absorbent material.
  • Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms
 
Volume:
  • 10mL in transport media
 
Storage/Transport:  
Unacceptable:
  • Leaking specimen, mixed isolate, or stool contaminated with urine
 
Specimen Stability:
  • Stool stable at 2-8 °C for up to 4 days
 
Reference Interval:  
Reportable Disease:  
Comments:
  • Test performed at Nebraska Medicine
 
Revised: 7/25/2024 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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