NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see
NUlirt Guide).
Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing
will not be performed without all required information.
Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.
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| Test: |
Mycobacterium tuberculosis Complex Genotyping
|
| Synonym: |
- MTB genotyping
- TB
- Mycobacterium tuberculosis
- MTBC
- Spoligotyping
|
| Method: |
Whole genome sequencing |
| Availability: |
Monday - Friday |
| Specimen: |
Pure isolate in liquid (MGIT) or solid media (Lowenstein-Jensen or Middlebrook) |
| Collection Device: |
- Isolate on media slant or plate
- Seal with Parafilm™ or tape
- Test subject to CLIA regulations and requires 2 patient identifiers on container and requisition forms
|
| Volume: |
Submitting laboratory should keep 1 mL of MGIT and send the remaining or a subculture on solid media |
| Storage/Transport: |
- For shipping of isolates, see Category A instructions
- Specimen Packaging & Shipping - NPHL
- NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens
- Seal tubes with Parafilm™ or tape and place in biohazard bag with absorbant material
|
| Unacceptable: |
Mixed isolates, clinical specimens (ie. sputum, BAL) |
| Specimen Stability: |
Stable at ambient air 15°-25° C |
| Reference Interval: |
|
| Reportable Disease: |
|
| Comments: |
Test performed at Michigan Department of Community Health, TB laboratory, Lansing, MI 48906 |
| Revised: |
3/5/2024 |
If you have questions about proper specimen collection, please call
NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.
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