Brucella species  

 
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test: Brucella species  
Synonym: Brucellosis, Brucella suis, Brucella melitensis, Brucella canis, Brucella abortus  
Method: CDC Laboratory Response Network approved methods  
Availability:
  • Contact the NPHL emergency pager (402) 888-5588 for guidance if Bacillus anthracis cannot be ruled out in the laboratory
  • Perform only required LRN protocol to rule out and refer potentially hazardous pathogens according to LRN-B guidlines NPHLBenchGuide_FINAL20131221.pdf
 
  • Test performed Monday-Friday
  • Turn-around time: 1-10 days
 
Specimen: Isolates, clinical specimens (blood, serum, bone marrow, body fluids, tissues), environmental samples  
Collection Device:
  • Isolate on media slant or plate
  • Seal with Parafilm™ or tape
  • Test subject to CLIA regulations and requires 2 patient identifiers on container and requisition forms
 
Volume:
  • Requested volume for liquid clinical samples: 1 mL
  • Minimum volume for liquid clinical samples: 0.5 mL
 
Storage/Transport:
  • Category A packaging according to DOT and IATA Category A UN2815 guidlenes
  • Category A packages can only be transported by a Category A certified courier
  • https://www.nphl.org/index.cfm/specimen-packaging-shipping/
  • NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens
 
Unacceptable:  
Specimen Stability:  
Reference Interval:  
Reportable Disease:  
Comments: Contact the NPHL emergency pager before sending specimen (402) 888-5588  
Revised: 3/6/2024 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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