Burkholderia pseudomallei  

 
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test: Burkholderia pseudomallei  
Synonym: Meliodosis, Whitmore's disease  
Method:
  • CDC Laboratory Response Network methodology
 
Availability:
  • Contact the NPHL emergency pager 402.888.5588
 
  • Test performed Monday-Friday
  • Turnaround time 1-7 days
 
Specimen:
  • Isolates, whole blood, serum, envirnomental samples
 
Collection Device:
  • Isolate on media slant or plate
  • Seal with Parafilm or tape
 
  • Test subject to CLIA regulations and requires  patient identifiers on container and requisition forms
 
Volume:
  • Requested volume for liquid clinical samples: 1 mL
  • Minimum volume for liquid clinical samples: 0.5 mL
 
Storage/Transport:
  • Category A packaging according to DOT and IATA Category A UN2814 guidlines
  • Category A packages can only be transported by a Category A certified courier
  • https://www.nphl.org/index.cfm/specimen-packaging-shipping/
  • Seal tubes with Parafilm or tape and place in biohazard bag with absorbent material
  • NUlirt packing list or approprate paperwork (completed in entirety) must accompany specimens
 
Unacceptable:
  • Leaking container, inapproprate specimen type, insufficient volume
 
Specimen Stability:
  • Stable at ambient air 15-25 °C
 
Reference Interval:  
Reportable Disease:
  • Report positive Burkolderia pseudomallei immediately to local or state health department.
  • Refer to Nebraska DHHS Title 173, Communicable Diseases.
 
Comments:
  • Contact NPHL emergency pager before sending specimen (402) 888-5588
 
Revised: 3/13/2024 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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