Bacillus anthracis  

 
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test: Bacillus anthracis  
Synonym: anthrax  
Method: CDC Laboratory Response Network approved methods  
Availability:
  • Contact the NPHL emergency pager (402) 888-5588 for guidance if Bacillus anthracis cannot be ruled out in the laboratory
  • Perform only required LRN protocol to rule out and refer potentially hazardous pathogens according to LRN-B guidlines NPHLBenchGuide_FINAL20131221.pdf
 
Specimen: Isolates, whole blood (EDTA or sodium citrate), serum, plasma, pleural fluid, respiratory specimens (transtracheal aspirates, bronchial lavage), CSF, clinical swabs, environmental samples  
Collection Device:
  • Isolate on media slant or plate.
  • Seal with Parafilm or tape.
  • Test subject to CLIA regulations and requires 2 patient identifiers on container and requisition forms
 
Volume:
  • Requested volume for liquid clinical samples: 1.0 mL
  • Minimum liquid clinical sample: 0.5 mL
 
Storage/Transport:
  • Category A packaging according to DOT and IATA Category A UN2814 guidlines.
  • Category A packages can only be  transported by a Category A certified courier. 
  • https://www.nphl.org/index.cfm/specimen-packaging-shipping/
  • NUlirt packing list or appropiate paperwork (completed in entirety) must accompany specimens
 
Unacceptable:  
Specimen Stability: Stable at ambient air 14o-25o C  
Reference Interval:  
Reportable Disease:  
Comments: Contact NPHL emergency pager before sending specimen (402) 888-5588  
Revised: 3/6/2024 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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