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Rubella RT-PCR or Detection (CDCSO)  

 
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test: Rubella RT-PCR or Detection  
Synonym:
  • Rubella detection, Rubella molecular, VPD, Vaccine preventable disease, German measles, three-day measles
 
Method:
  • RT-PCR
 
Availability:
  • Monday - Friday
  • Turnaround time PCR: 2 days
  • Turnaround time genotyping: 10 days
  • Submit all specimens through the state health departments, NOT directly to the Minnesota Department of Health
 
Specimen:
  • Throat swab
  • Nasopharyngeal aspirate or swab
 
Collection Device:
  • Store swabs/aspirates in 2 mL viral transport media
  • Seal with Parafilm™ or tape
  • Test subject to CLIA regulations and requires 2 patient identifiers on container and requisition forms
 
Volume:
  • 250 µL
 
Storage/Transport:  
Unacceptable:
  • Samples stored incorrectly
 
Specimen Stability:
  • Specimen stable at 4 °C up to 24h refrigerated
  • Freeze after 24h
 
Reference Interval:  
Reportable Disease:  
Comments:
  • Genotyping will be performed on all PCR positive specimens unless otherwise indicated as a part of a larger outbreak
  • Test performed at the Minnesota Department of Health, Public Health Laboratory, St. Paul, MN
  • Submit all specimens through NPHL, NOT directly to the CDC
 
Revised: 4/23/2024 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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