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Mumps Virus RT-PCR or Genotyping (CDCSO)  

 
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test: Mumps Virus RT-PCR or Genotyping  
Synonym:
  • Mumps detection, mumps molecular, VPD, vaccine preventable disease
 
Method:
  • RT-PCR
 
Availability:
  • Monday - Friday
  • Turnaround time 2 days for PCR, 10 days for genotyping
 
Specimen:
  • Buccal swab
  • Nasal swab
  • Urine
 
Collection Device:
  • Swabs in 2 mL viral transport media
  • Urine specimens in sterile cup
  • Seal tubes with Parafilm™ or tape and place in biohazard bag with absorbent material
  • Test subject to CLIA regulations and requires 2 patient identifiers on container and requistion forms
 
Volume:
  • 250 µL
 
Storage/Transport:
  • For shipping, see Category B instructions
  • NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.
  • Submit all specimens through the state or local health departments, NOT directly to the Minnesota Department of Health
 
Unacceptable:
  • Samples stored incorrectly
 
Specimen Stability:
  • Stable at 4 °C for 24 hours
  • Freeze at -70 °C after 24 hours
 
Reference Interval:  
Reportable Disease:  
Comments:
  • Test performed at the Minnesota Department of Health, Public Health Laboratory, St. Paul, MN
  • Submit all specimens through NPHL, NOT directy to Minnesota
  • Genotyping will be performed on all PCR positive specimens unless otherwise indicated as a part of a larger outbreak
 
Revised: 4/10/2024 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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