Botulism Laboratory Confirmation  

IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.


Test: Botulism Laboratory Confirmation
Synonym: Botulinum toxin, Clostridium botulinum  
  • See CDC Test Directory for methodology, specimen types, collection/stability, labeling, volume, interferences/limitations, and turn-around times
  • CDC Test Code CDC-10132
  • Monday – Thursday
  • Submit all specimens for CDC through the state and local health departments, NOT directly to the CDC. CDC does not accept routine shipments on weekends or holidays. Therefore, shipto NPHL Monday through Thursday to guarantee arrival at the CDC by Friday
Collection Device:  


  • For shipping, see Category B shipping instructions
  • Seal screw-top tubes with Parafilm™ or tape and place in biohazard bag with absorbent material
  • NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens
Specimen Stability:  
Reference Interval:  
Reportable Disease:  
  • Test Performed at the CDC
  • Submit all specimens to the CDC through NPHL, NOT directly to the CDC

Revised: 3/6/2024 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.