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Coronavirus-19 and Influenza Multiplex Assay RT-PCR (NCOVFL)  

 
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test: CoVID-19 and Influenza Multiplex Assay RT-PCR
  • Pre-approval needed from state or local health department
  • Send approval to NPHL@unmc.edu or call (402) 559-9444
  • NUlirt order: NCOVFL
 
Synonym:
  • Specific detection of 2019-nCoV
  • SARS-CoV2
  • Novel coronavirus
  • Influenza A
  • Flu A
  • Influenza B
  • Flu B
 
Method:
  • Qualitative nucleic acid amplified real time RT-PCR (RT-PCR) assay
 
Availability:
  • Test performed Monday – Friday
  • Turnaround time 24-48h
 
Specimen:
  • Nasopharyngeal swab
  • Nasal Swab
 
Collection Device:
  • Viral Transport Media  (VTM, UTM, VCM or M4)
  • Saline
  • Specimens should be collected only on mini-tipped swabs with a synthetic tip (such as polyester or Dacron®) with aluminum or plastic shafts
  • Do not use calcium alginate swabs or swabs with wooden shafts, as they may contain substances that inactivate some viruses and inhibit PCR testing
  • Seal with Parafilm™ or tape
  • Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms
 
Volume:
  • 1-3 mL viral transport media
 
Storage/Transport:  
Unacceptable: • Specimens not kept at 2-8°C (≤3 days) or frozen at -20°C or below. • Inappropriate specimen type. • Insufficient specimen volume. • Swabs submitted with calcium alginate or cotton tips with wooden shafts are not acceptable.  
Specimen Stability:
  • Stable at Refrigerated 2-8°C < 72hr; Frozen -20°C  >72h, stable frozen for 30 days
 
Reference Interval:  
Reportable Disease:  
Comments:      
Revised: 7/26/2024 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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