Rotavirus Antigen  

IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.


Test: Rotavirus Antigen  
Synonym: Rota  
Method:  EIA  
Availability: Test performed daily, results same day  
Specimen: Stool or rectal swab from infants with visible stoolations and required 2 patient identifiers on specimen container and requisition forms  
Collection Device: Fresh stool collected without preservative.  Do not use transport media, formalin, PVA or SAF.
Rectal swabs are acceptable from infants only. Do not use wire-shafted swabs.
Specimen Labeling: Test subject to CLIA regul  
Volume: 1.0 mL stool minimum.  

Prior to and during shipment: Refrigerate 4-8°C or Frozen
Shipping instructions, including specimen-handling requirements during transport:
All screw-top tubes must be sealed with parafilm or tape.  Vacutainer tubes do not require parafilm or tape to seal.
Package and ship as UN3373 Category B, Biological Substance. Place in biohazard bag with absorbent material. Refrigerated specimens should be shipped on frozen gel-packs. Frozen specimens should be shipped on dry ice.  Transport by NPHL ground courier (NPHL brown shipper not required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup.  
See instructions and shipping address:

Unacceptable: Specimens received in diapers.  
Specimen Stability: Stable at Refrigerated temperature 2-8°C for 3 days, Frozen up to 1 week.  
Reference Interval: Negative  
Reportable Disease: Positive results are reported to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases  
Comments: Pre-approval needed from state or local health department.  Send approval to or call (402) 559-9444.
Supplemental Forms Required – Nulirt packing list or
NPHL Test Order Form (completed in entirety) must accompany the specimen. Virus is available for testing on the Gastrointestinal Pathogen Panel (GIP). If ordered in combination with the GIP it is considered a duplicate test.  
Revised: 8/27/2019 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.