Reportable Diseases, Isolate Confirmation  

IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.


Test: Bacterial Organism Identification for Confirmation of Reportable Disease Isolates  
Method: Standard reference procedures for bacterial identification  
Availability: Monday through Friday by 0900  
Specimen: Submit actively-growing isolated organism in pure culture on agar slant  
Collection Device:  
Storage/Transport: Submit organism in NPHL Triple Pack at room temperature. Specimen must be shipped via NPHL ground courier or Federal Express. Call NPHL at 402.559.2440 or 866.290.1406 for pickup.  
Unacceptable: Nonviable organism, mixed culture, leaking container  
Specimen Stability:  
Reference Interval: Full identification of clinically significant isolates  
Reportable Disease:  
Comments: Indicate source of organism, identification test results, suspected infection and any other pertinent information. For AFB mycobacterium isolates, see "AFB/Mycobacterium Identification." See "Escherichia coli (Hemorrhagic) Culture, Stool" for confirmation of E. coli 0157:H7 isolates. 
Revised: 2/13/2012 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.