HIV Antigen/Antibody Screen  

IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.


Test: HIV Antigen/Antibody Panel  
Synonym: HIV ELISA screen, HIV screen 
Method: Multiplex flow immunoassay  
Availability: Daily, results in 24 hours  
Specimen: Blood  
Collection Device: 6 ml SST (Gold) tube, 6ml Clot (Red) tube, or 5 ml EDTA (Lavender) tube  
Volume: Minimum 0.5 ml serum or plasma  
Storage/Transport: Refrigerated. If transport of specimen not on the same day as collected, centrifuge and aliquot the serum or plasma into a clean tube.  
Unacceptable: Samples in sodium citrate, other body fluids, hemolyzed specimens, specimen on cells > 24 hours  
Specimen Stability: After separation from cells: ambient if < 24 hours; refrigerated if > 24 hours up to 14 days; frozen if > 14 days.  
Reference Interval: Nonreactive  
Reportable Disease: Reactive is reportable 
Comments: Reflexed to HIV 1,2 Antibody confirmation testing if antigen/antibody screen is reactive.

Detection of HIV-1 p 24 antigen in the absence of detectable HIV antibody may indicate an acute infection. Confirmation testing is performed using the quantitative HIV RNA assay.

All HIV-2 antibody reactive will be confirmed by the CDC. 
Revised: 2/28/2017 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.