Respiratory Syncytial Virus Direct Antigen Detection  

IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.


Test: Respiratory Syncytial Virus Direct Antigen Detection  
Synonym: RSV Direct 
Method: Enzymatic Immunoassay  
Availability: Daily; routine results within 4 hours and stat results within 1 hour of specimen receipt in the Microbiology Laboratory  
Specimen: Nasopharyngeal wash, aspirate, or swab  
Collection Device: Place specimen in viral transport media  
Volume: 3.0 mL wash (Min: 2.0 mL)  
Storage/Transport: Refrigerate; must be received by laboratory within 72 hours  
Unacceptable: BAL,sputum, smears, bronchial washings  
Specimen Stability: Refrigerated, < 72 hours  
Reference Interval: Negative  
Reportable Disease:  
Comments: A negative screen does not rule out RSV. 
Revised: 7/11/2011 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.