Test: | Hepatitis B Surface Antibody Order in NUlirt or complete NPHL Test Order Form |
Synonym: | Antibody to Hepatitis B Surface Antigen; Anti-HBs; Australian Antigen |
Method: | Chemiluminescence |
Availability: | Daily, Results in 24h |
Specimen: | Blood |
Collection Device: | One 6mL SST (Gold) tube or One 5mL EDTA (Lavender) tube. Specimen Labeling: Test subject to CLIA regulations and requires 2 patient identifiers and specimen source on container. |
Volume: | 1mL serum or plasma (0.5mL minimum) |
Storage/Transport: | Prior to and during shipment: Refrigerate 2-8°C. If sample is not transported to lab the same day as collected, aliquot the serum or plasma. To aliquot: Centrifuge the sample and transfer into a clean tube, label as "serum" or "plasma." |
Unacceptable: | Vacutainers not separated, heparinized plasma |
Specimen Stability: | After separation from cells: Ambient: <8 hours; Refrigerated: 72h if separated, 24h if gel tube; Frozen: 1y (avoid more than 2 freeze/thaw cycles) |
Reference Interval: | Protective level is >10 mIU/mL. |
Reportable Disease: | Not applicable |
Comments: | Pre-approval needed from state or local health department. Send approval to NPHL@unmc.edu or call (402) 559-9444. Biotin Therapy may invalidate results. Some assays may exhibit interference when samples are collected from patients consuming higher than recommended doses of biotin or supplements (including prenatal multivitamins). Simple daily supplementation, recommended dosage, is not problematic. It is recommended that patients be asked if they are consuming high-dose biotin or biotin containing supplements. Patients should be cautioned to stop using biotin-containing supplements at least 72 hours prior collection. NUlirt packing list or NPHL Test Order Form (completed in entirety) must accompany the specimen. |
Revised: | 6/11/2021 |