Hepatitis B Surface Antibody  

IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.


Test: Hepatitis B Surface Antibody
Order in NUlirt or complete NPHL Test Order Form   
Synonym: Antibody to Hepatitis B Surface Antigen; Anti-HBs; Australian Antigen  
Method: Chemiluminescence  
Availability: Daily, Results in 24h  
Specimen: Blood  
Collection Device: One 6mL SST (Gold) tube or One 5mL EDTA (Lavender) tube.
Specimen Labeling: Test subject to CLIA regulations and requires 2 patient identifiers and specimen source on container.  
Volume: 1mL serum or plasma (0.5mL minimum)  

Prior to and during shipment:  Refrigerate 2-8°C. If sample is not transported to lab the same day as collected, aliquot the serum or plasma. To aliquot: Centrifuge the sample and transfer into a clean tube, label as "serum" or "plasma."
Shipping instructions, including specimen-handling requirements during transport:  Seal screw-top tubes with Parafilm™ or tape and place in biohazard bag with absorbent material.
Place specimen inside leak proof secondary container (biohazard bag and if transporting by air, use Tyvek™ Envelope) with adsorbent material. The secondary container must be packed inside an approved Category B shipping container filled with frozen gel-packs to keep specimen refrigerated. Position NUlirt packing list or NPHL Test Order form between secondary container and box lid. Seal with tape.  Label outer box as UN3373 Category B, Biological Substance. Transport by NPHL ground courier. If courier not available, ship by FedEx, UPS, or USPS (1st Class, Priority or Express Mail). Follow appropriate DOT/IATA Regulation Guidelines.  For assistance, call NPHL State Training Coordinator at (402) 559-3590.  
See instructions and shipping address: http://nphl.org/

NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.

Unacceptable: Vacutainers not separated, heparinized plasma  
Specimen Stability: After separation from cells: Ambient: <8 hours; Refrigerated: 72h if separated, 24h if gel tube; Frozen: 1y (avoid more than 2 freeze/thaw cycles)  
Reference Interval: Protective level is >10 mIU/mL.  
Reportable Disease: Not applicable  
Comments: Pre-approval needed from state or local health department.  Send approval to NPHL@unmc.edu or call (402) 559-9444.

Biotin Therapy may invalidate results. Some assays may exhibit interference when samples are collected from patients consuming higher than recommended doses of biotin or supplements (including prenatal multivitamins). Simple daily supplementation, recommended dosage, is not problematic. It is recommended that patients be asked if they are consuming high-dose biotin or biotin containing 

supplements. Patients should be cautioned to stop using biotin-containing supplements at least 72 hours prior collection.

NUlirt packing list or NPHL Test Order Form (completed in entirety) must accompany the specimen.  
Revised: 6/11/2021 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.