Norovirus RNA Direct Detection (NVOBD) (NPHLBK)  

IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.


Test: Norovirus RNA Direct Detection
  • Pre-approval needed from state or local health department
  • Send documented approval to or call (402) 559-9444
  • NUlirt orders: NVOBD and NPHLBK
Synonym: Norwalk virus, Human Calicivirus PCR, Norovirus PCR  
  • Cepheid Gene Xpert Norovirus Assay, a real-time RT-PCR assay
  • Test performed Monday – Friday
  • Turnaround time 24-48 hours
  • Stool 
Collection Device:
  • Sterile Container
  • Seal with Parafilm™ or tape
  • Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms
  • Minimum 1 mL
  • Stool stored at room temperature >8 hours and
  • Stool contaminated with urine
Specimen Stability:
  • Ambient, 8 hours
  • Refrigerated, 3 days
  • Frozen, 1 month
Reference Interval:  
Reportable Disease:  
  • Testing performed on suspected outbreak specimens after state approval
Revised: 4/10/2024 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.