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Norovirus RNA Direct Detection (NVOBD) (NPHLBK)  

 
NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test: Norovirus RNA Direct Detection
  • Pre-approval needed from state or local health department
  • Send documented approval to NPHL@unmc.edu or call (402) 559-9444
  • NUlirt orders: NVOBD and NPHLBK
   
Synonym: Norwalk virus, Human Calicivirus PCR, Norovirus PCR  
Method:
  • Cepheid Gene Xpert Norovirus Assay, a real-time RT-PCR assay
 
Availability:
  • Test performed Monday – Friday
  • Turnaround time 24-48 hours
 
Specimen:
  • Stool 
 
Collection Device:
  • Sterile Container
  • Seal with Parafilm™ or tape
  • Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms
 
Volume:
  • Minimum 1 mL
 
Storage/Transport:  
Unacceptable:
  • Stool stored at room temperature >8 hours and
  • Stool contaminated with urine
 
Specimen Stability:
  • Ambient, 8 hours
  • Refrigerated, 3 days
  • Frozen, 1 month
 
Reference Interval:  
Reportable Disease:  
Comments:
  • Testing performed on suspected outbreak specimens after state approval
 
Revised: 4/10/2024 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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