NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see
NUlirt Guide).
Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing
will not be performed without all required information.
Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.
BACK | PRINT
Test: |
Shiga toxin-producing Escherichia coli
- No pre-approval needed
- NUlirt order:
- Isolate: NPHLBK
- Stool: HECCU
|
Synonym: |
- E. coli, O157:H7, Shigatoxin positive, non-O157 E. coli, STEC, hemorragic E. coli, Shiga toxin-positive, E coli
|
Method: |
- Stool: Culture
- Isolate: Banking and whole genome sequencing (WGS)
|
Availability: |
- Test performed Monday - Friday
- Turnaround time 1 - 7 days
|
Specimen: |
|
Collection Device: |
- Stool specimens should be collected in Cary Blair or Enteric Plus transport meida according to manufacturer's instructions
- If fresh stool is received, it should be placed into transport media within 2 hours of collection
- Ratio of stool to Enteric Plus is 2:15 (0.5 mL stool to 3.75 mL Enteric Plus liquid media)
- Isolates should be on a plate or slant
- Seal screw-top tubes with Parafilm™ or tape and place in biohazard bag with absorbent material.
- Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms
|
Volume: |
- Stool samples minimum: 1 mL
|
Storage/Transport: |
|
Unacceptable: |
- Leaking specimen, mixed isolates, or stool contaminated with urine
|
Specimen Stability: |
- Stool stable at 2-8 °C for up to 4 days
- Isolates stable at ambient air 15-25 °C
|
Reference Interval: |
|
Reportable Disease: |
|
Comments: |
- Stool culture performed at Nebraska Medicine
- Banking and Whole Genome sequencing performed at NPHL
- Test results are to be used for epidemiological and infection control purposes only
|
Revised: |
7/2/2024 |
If you have questions about proper specimen collection, please call
NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.
BACK