Shigella spp. (NPHLBK) (ORGSS)  

IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.


Test: Shigella spp.
  • No pre-approval needed
  • Forward all species except Shigella sonnei (unless NE DHHS requests)
  • NUlirt order: 
    • NPHLBK for isolates
    • ORGSS for stool
  • Shigella flexneri, Shigella sonnei, Shigella dysenteriae, Shigellosis
  • For stool: 
    • Culture
  • For isolate:
    • Banking
    • Whole genome sequencing
  • Test performed Monday-Friday
  • Turnaround time 1-7 days
  • Pure isolate or
  • Stool
Collection Device:
  • Stool: Cary Blair or Enteric Plus transport media
    • Fresh stool should be plaed in media within 2 hours or manufacturer's instructions
    • Seal with Parafilm™ or tape
  • Isolate can be on a plate or slant
    • Seal with Parafilm™ or tape
  • Test subject to CLIA regulations and requires 2 patient identifiers on container and requisition forms
  • Stool sample, minimum 1 mL
  • Leaking specimens
  • Mixed isolates
  • Stool contaminated with urine
Specimen Stability:
  • Isolates, stable ambient air 15-25 °C
  • Stool in transport media stable at 2-8 °C up to 4 days
Reference Interval:  
Reportable Disease:  
  • Isolation of Shigella species from stool performed at Nebraska Medicine
  • Test results are to be used for epidemiological and infection control purposes only
Revised: 5/21/2024 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.