IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.


Test: Mpox
  • Contact NPHL emergency pager (402) 888-5588
  • Monkeypox, Non-variola orthopox, Orthopox, Orthopoxvirus
Method: CDC Laboratory Response Network methods  
  • Monday - Friday
  • Turnaround time 1-7 days
  • Dry swabs from lesion
  • Test subject to CLIA regulations and requires 2 patient identifiers on container and requisition forms
  • Seal tubes with Parafilm™ or tape and place in biohazard bag with absorbent material
Collection Device:  
  • Collect 2 swabs from two different locations for a total of four swabs
Specimen Stability:
  • Freeze or refrigerate promptly after collection
  • Store frozen dry swabs up to 60 days
  • Freezing is strongly recommended, however, if no freezer is available, refrigerate samples up to 7 days
Reference Interval:  
Reportable Disease:  
  • Contact NPHL emergency pager before sending specimen (402) 888-5588
Revised: 4/3/2024 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.