IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.


Test: Ebolavirus
  • Pre-approval needed from state or local health department and the Centers for Disease Control and Prevention (CDC).
  • Call NPHL emergency pager (402) 888-5588 before sending specimen
  • Ebola hemorrhagic fever
  • Zaire Ebolavirus
  • Sudan virus
  • Bundibugyo virus
  • Reston virus
  • Tai Forest virus, Zaire Ebolavirus Makona
  • Zair Ebolavirus Mayinga
Method: CDC Laboratory Response Network methodology  
  • Test performed Monday - Friday
  • Tournaround time 12 hours
  • Suspected Zire strains: Blood (whole blood, serum, plasma), urine if paired with blood
  • Suspected Sudan strains: Whole blood in EDTA (lavendar) tube
Collection Device: Test subject to CLIA regulations and requires 2 patient identifiers on container and requisition forms  
  • Requested volume: 4 mL
  • Minimum volume: 0.2 mL
  • For shipping instructions, see Category A
  • Call NPHL emergency pager (402) 888-5588  for guidance when shipping potential Ebolavirus


  • Leaking container, inappropriate speciment type, insufficient volume
Specimen Stability: Stable at 2-8ºC for 7 days Serum, plasma, and urine may be frozen at -70ºC if delay is anticipated  
Reference Interval:  
Reportable Disease:  
Comments: Contact NPHL emergency pager before sending specimen (402) 888 - 5588  
Revised: 3/18/2024 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.