AFB Mycobacterium tuberculosis Complex Genotyping (TBGEN)  

IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.


Test: Mycobacterium tuberculosis Complex Genotyping  
Synonym: MTB genotyping, TB, Mycobacterium tuberculosis, MTBC, Spoligotyping  
Method: Whole genome sequencing  
  • Pre-approval required from state or local health department
Specimen: Pure isolate in liquid (MGIT) or solid media (Lowenstein-Jensen or Middlebrook)  
Collection Device:
  • Isolate on media slant or plate
  • Seal with Parafilm™ or tape. 
  • Test subject to CLIA regulations and requires 2 patient identifiers on container and requisition forms
Volume: Submitting laboratory should keem 1 mL of MGIT and end the remaining or a subculture on solid media  
  • For shipping of isolates, see Category A instructions
  • NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens
  • Seal tubes with Parafilm™ or tape and place in biohazard bag with absorbent material
Unacceptable: Mixed isolates, clinical specimens (ie. sputum, BAL)  
Specimen Stability: Stable at ambient air 15o-25o C  
Reference Interval:  
Reportable Disease:  
Comments: Test performed at Michigan Department of Community Health, TB laboratory, Lansing, MI 48906  
Revised: 3/5/2024 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.