IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to
NPHL@unmc.edu or call (402) 559-9444.
NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see
NUlirt Guide).
Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing
will not be performed without all required information.
Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.
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Test: |
Burkholderia pseudomallei |
Synonym: |
Meliodosis, Whitmore's disease |
Method: |
- CDC Laboratory Response Network methodology
|
Availability: |
- Contact the NPHL emergency pager 402.888.5588
- Test performed Monday-Friday
- Turnaround time 1-7 days
|
Specimen: |
- Isolates, whole blood, serum, envirnomental samples
|
Collection Device: |
- Isolate on media slant or plate
- Seal with Parafilm or tape
- Test subject to CLIA regulations and requires patient identifiers on container and requisition forms
|
Volume: |
- Requested volume for liquid clinical samples: 1 mL
- Minimum volume for liquid clinical samples: 0.5 mL
|
Storage/Transport: |
- Category A packaging according to DOT and IATA Category A UN2814 guidlines
- Category A packages can only be transported by a Category A certified courier
- https://www.nphl.org/index.cfm/specimen-packaging-shipping/
- Seal tubes with Parafilm or tape and place in biohazard bag with absorbent material
- NUlirt packing list or approprate paperwork (completed in entirety) must accompany specimens
|
Unacceptable: |
- Leaking container, inapproprate specimen type, insufficient volume
|
Specimen Stability: |
- Stable at ambient air 15-25 °C
|
Reference Interval: |
|
Reportable Disease: |
- Report positive Burkolderia pseudomallei immediately to local or state health department.
- Refer to Nebraska DHHS Title 173, Communicable Diseases.
|
Comments: |
- Contact NPHL emergency pager before sending specimen (402) 888-5588
|
Revised: |
3/13/2024 |
If you have questions about proper specimen collection, please call
NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.
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