Burkholderia pseudomallei  

 
NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test: Burkholderia pseudomallei
  • Contact the NPHL emergency pager (402) 888-5588 for guidance if Burkholderia pseudomallei cannot be ruled out in the laboratory
  • Perform only required LRN protocol to rule out and refer potentially hazardous pathogens according to LRN-B guidlines 
  • NPHLBenchGuide_FINAL20131221.pdf
 
Synonym:
  • Meliodosis
  • Whitmore's disease
 
Method:     CDC Laboratory Response Network approved methods      
Availability:
  • Test performed Monday-Friday
  • Turnaround time 1-7 days
 
Specimen:
  • Isolates
  • Clinical Specimens (blood, serum, bone marro, fbody fluids, tissues)
  • Environmental Samples
 
Collection Device:
  • Isolate on media slant or plate
  • Seal with Parafilm™ or tape
  • Test subject to CLIA regulations and requires  patient identifiers on container and requisition forms
 
Volume:
  • Requested volume for liquid clinical samples: 1 mL
  • Minimum volume for liquid clinical samples: 0.5 mL
 
Storage/Transport:
  • Category A packaging according to DOT and IATA Category A UN2815 guidlenes
  • Category A packages can only be transported by a Category A certified courier
  • https://www.nphl.org/index.cfm/specimen-packaging-shipping/
  • NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimen
 
Unacceptable: Leaking container, inapproprate specimen type, insufficient volume  
Specimen Stability:
  • Stable at ambient air 15-25 °C
 
Reference Interval:  
Reportable Disease:
  • Report positive Burkolderia pseudomallei immediately to local or state health department.
  • Refer to Nebraska DHHS Title 173, Communicable Diseases.
 
Comments: Contact NPHL emergency pager before sending specimen (402) 888-5588  
Revised: 3/13/2024 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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