Arbovirus Antibodies, IgG and IgM, Serum or CSF  

 
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test: Arbovirus Antibodies, IgG and IgM, Serum or CSF
Order in NUlirt or complete NPHL Test Order Form with Supplemental Forms  
http://nphl.org/documents/2021_CDC_DASH_50.34.pdf   
Synonym: Arbovirus, Arbo-Serology for Barmah Forest virus (BFV), Chikungunya virus (CHIKV), Dengue virus (DENV), Eastern equine encephalitis virus (EEEV), Heartland virus (HRTV), Jamestown Canyon virus (JCV), Japanese encephalitis virus (JEV), La Crosse Encephalitis virus (LACV), Mayaro virus (MAYV), Murray Valley encephalitis virus (MVEV), O'nyong nyong virus (ONNV), Powassan virus (POWV), Ross River virus (RRV), Saint Louis encephalitis virus (SLEV), Sindbis virus (SINV), Snowshoe hare virus (SSHV), Tahyna virus (TAHV), Venezuelan equine encephalitis virus (VEEV),West Nile virus (WNV), Western equine encephalitis virus (WEEV), Yellow fever virus (YFV), Zika virus (ZIKV).  
Method:  
Availability: Note: Contact the NPHL for guidance if arbovirus is suspected.  

For more information on Eastern Equine Encephalitis, California Encephalitis, St. Louis Encephalitis and Western Equine Encephalitis, please review the current clinical guidelines for testing at: https://ltd.aruplab.com/Tests/Pub/2001594 or https://ltd.aruplab.com/Search/SearchOrderables?search=arbo

For more information on Dengue, please review the current clinical guidelines for testing at: https://www.testmenu.com/nebraska/search?searchinput=Dengue+Fever+IgG+and+IgM+Antibodies 
For more information on Zika (IgM only), please review the current clinical guidelines for testing at:
https://ltd.aruplab.com/Search/SearchOrderables?search=zika 

For more information on all other arbovirus, please review the current clinical guidelines for testing at: https://www.cdc.gov/laboratory/specimen-submission/detail.html?CDCTestCode=CDC-10282

Submit all specimens through the state health departments, NOT directly to the CDC or ARUP. CDC does not accept routine shipments on weekends or holidays. Therefore, ship to NPHL on Monday – Thursday, to guarantee arrival at the CDC by Friday.  
Specimen: See links above.  
Collection Device: See links above.
Specimen Labeling: Test subject to CLIA regulations and requires 2 patient identifiers and specimen source on container.  
Volume: Serum: 1mL;   Minimum: 0.5mL  
Storage/Transport:

Centrifuge and aliquot serum into sterile leak proof tube, within 2h of collection.  
Prior to and during shipment:  Store serum or CSF at 2-8°C.

Shipping instructions, including specimen-handling requirements during transport: Seal capped tubes with Parafilm™ or tape and place in biohazard bag with absorbent material.

Place specimen inside leak proof secondary container (biohazard bag and if transporting by air, use Tyvek™ Envelope) with adsorbent material. The secondary container must be packed inside an approved Category B shipping container filled with frozen gel-packs to keep specimen refrigerated. Position NUlirt packing list or NPHL Test Order form between secondary container and box lid. Seal with tape.  Label outer box as UN3373 Category B, Biological Substance. Transport by NPHL ground courier. If courier not available, ship by FedEx, UPS, or USPS (1st Class, Priority or Express Mail). Follow appropriate DOT/IATA Regulation Guidelines.  For assistance, call NPHL State Training Coordinator at (402) 559-3590. 

See instructions and shipping address: http://nphl.org/

NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.

 
Unacceptable: Severely lipemic, icteric or hemolyzed samples; heat-inactivated serum; samples that have undergone multiple freeze thaw cycles. Hemolysis can cause non-specific binding in serological tests and can have an effect on laboratory results.  
Specimen Stability: 2-8°C for 7d, if >7d, ≤-20°C. Avoid repeated freeze-thaw cycles.  
Reference Interval: Negative - No significant level of detectable arbovirus antibody.
Positive - IgM antibody to arbovirus detected and reflexed for confirmation testing.   
Reportable Disease: A positive result is reportable as Arbovirus infection under Title 173, report within 7d. Refer to Nebraska DHHS Title 173, Communicable Diseases.   
Comments: Pre-approval needed from state or local health department.  Send documented approval to NPHL@unmc.edu or call (402) 559-9444.

Test performed at the CDC or ARUP. After approval from local or state epi, submit specimens through the NPHL, NOT directly to a reference lab. NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.  
Revised: 7/15/2021 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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