Syphilis, Treponemal Antibody Fluorescent, IgG   

 
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test: Syphilis, Treponemal Antibody Fluorescent, IgG  
Order in NUlirt or complete NPHL Test Order Form  
Synonym: Fluorescent Treponemal Antibody(FTA), FTA-ABS  
Method: Indirect Fluorescent Antibody (IFA)  
Availability: Test performed on Wednesday by 0800; Results same day.  
Specimen: Serum  
Collection Device: One 6.0mL SST (Gold) tube.
To aliquot: Centrifuge the sample and transfer the serum into a clean tube. 

Please provide RPR result.  If no RPR result available, RPR will be performed prior to FTA.

Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms  
Volume: 2.0 mL serum; 0.5 mL minimum.  
Storage/Transport:

Prior to and during shipment: Centrifuge the sample and transfer the serum into a clean tube. Refrigerate at 2-8°C
Shipping instructions, including specimen-handling requirements during transport:  Seal screw-top tubes with Parafilm™ or tape and place in biohazard bag with absorbent material.

Place specimen inside leak proof secondary container (biohazard bag and if transporting by air, use Tyvek™ Envelope) with adsorbent material. The secondary container must be packed inside an approved Category B shipping container filled with frozen gel-packs to keep specimen refrigerated. Position NUlirt packing list or NPHL Test Order form between secondary container and box lid. Seal with tape.  Label outer box as UN3373 Category B, Biological Substance. Transport by NPHL ground courier. If courier not available, ship by FedEx, UPS, or USPS (1st Class, Priority or Express Mail). Follow appropriate DOT/IATA Regulation Guidelines.  For assistance, call NPHL State Training Coordinator at (402) 559-3590.  
See instructions and shipping address: http://nphl.org/

NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.
 

 
Unacceptable: Whole blood, improper specimen labels  
Specimen Stability: After separation from cells: Refrigerated: 48hr; Frozen: 1yr  (avoid repeated freeze/thaw cycles)  
Reference Interval: Nonreactive  
Reportable Disease: Report all positive Syphilis IgG results within 7d to the local or state health department, refer to Nebraska DHHS Title 173, Communicable Diseases.  
Comments: Pre-approval needed from state or local health department.  Send approval to NPHL@unmc.edu or call (402) 559-9444.

Please provide RPR result.  If no RPR result available, RPR will be performed prior to FTA.

NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.  
Revised: 6/21/2021 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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