Banking (including Confirmation) of Cryptosporidium or Cyclospora Positive Stools   

IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.


Test: Banking (including Confirmation) of Cryptosporidium or Cyclospora Positive Stools 
Order in NUlirt or complete NPHL Test Order Form  
Synonym: Bank  
Method: PCR  
Availability: Test performed Monday – Friday; Turnaround time 24h  
Specimen: CDC Fixatives/Preservatives Compatible with Molecular Detection  
Collection Device: Submit original container: Cary-Blair, Enteric Plus, TotalFix, Unifix, Ecofix, modified PVA (Zn- or Cu-based)
Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms  
Volume: 1-5mL for fresh stool; 10mL stool in Cary-Blair or Enteric Plus Maintenance media.  

Prior to and during shipment:  Ambient 15-25°C
Shipping instructions, including specimen-handling requirements during transport:  Seal screw-top tubes with Parafilm™ or tape and place in biohazard bag with absorbent material.

Place specimen inside leak proof secondary container (biohazard bag and if transporting by air, use Tyvek™ Envelope) ) with adsorbent material. The secondary container must be packed inside an approved Category B shipping container filled with dry ice to keep specimen frozen. Position NUlirt packing list or NPHL Test Order form between secondary container and box lid. Seal with tape.  Label outer box as UN3373 Category B, Biological Substance. Transport by NPHL ground courier. If courier not available, ship by FedEx, UPS, or USPS (1st Class, Priority or Express Mail). Follow appropriate DOT/IATA Regulation Guidelines.  For assistance, call NPHL State Training Coordinator at (402) 559-3590.  
See instructions and shipping address:

NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.

Unacceptable: Incorrect collection device containing Formalin i.e.) SAF, LV-PVA, ProtoFix  
Specimen Stability: Ambient, Submit with 72h  
Reference Interval: Negative  
Reportable Disease: Report positive Cryptosporidium or Cyclospora within 7d to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases.  
Comments: All stools positive for Cryptosporidium or Cyclospora should be submitted to NPHL Positive tests may include:
  1. Rapid card or lateral flow assay i.e.) Expect, Alere, ColorPac, TechLab, or other approved rapid card tests
  2. Stool Immunoassay i.e. EIA microplate
  3. Direct Fluorescent Antibody (DFA) or Direct Immunofluorscent Antibody (IFA)
  4. Staining & microscopy i.e.) modified acid fast stain
  5. PCR i.e.) Holigic, BioFire, BD Max, Verigene or other lab-developed test.
Revised: 6/11/2021 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.