Mumps Virus Culture  

IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.


Test: Mumps Virus Culture  
Method: Cell culture / Immunofluorescence  
Availability: Daily; results within 10 - 14 days  
Specimen: Saliva, Nasopharyngeal aspirate/washing, first-voided morning urine, CSF  
Collection Device: Viral transport medium (for saliva), nasopharyngeal viral transport swab, sterile, leak-proof container (for urine and CSF)  
Volume: No minimum  
Storage/Transport: Submit specimens at 2 - 8 °C, do not freeze  
Unacceptable: Dry swab, wood-shafted swab, calcium alginate swab, frozen specimen, leaking container  
Specimen Stability: After collection: ambient, 2 hours; refrigerated, 3 days; frozen, unacceptable  
Reference Interval: Culture negative for mumps virus  
Reportable Disease: A positive culture is reportable 
Comments: Specimens must be no more than 24 hours old and received at Regional Pathology Services no later than 1500 on Monday-Thursday. Due to specimen transport time, collection on Friday, Saturday, or Sunday is discouraged. Please call client services as soon as possible if sample is collected on Friday, Saturday, or Sunday. 
Revised: 2/13/2012 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.