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Comment: Sites submitting specimens for testing must be approved by the Nebraska Health
and Human Services System's Epidemiology Program within the Department of Regulation and Licensure.
A Special Microbiology Requisition or order must accompany the specimens.
Please call NPHL Client Services at 402-559-2440 or toll free 1-866-290-1406 for further clarification
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| TEST: |
Culture, Anaerobic and aerobic with Gram Stain |
| Synonym: |
Abscess or wound culture. anerobice culture |
| Lab Defined: |
Microbiology |
| Admin Section: |
Microbiology |
| Method: |
Test includes: Gram stain, isolation and identification of potential aerobic and anaerobic pathogens, and initiation of antibiotic susceptibility tests when indicated. |
| Availability: |
Daily; results in 24-72 hours. Culture showing no bacterial growth will be reported after 24 hours. Complete reports of cultures with anaerobic bacteria may take as long as 5 days after receipt of culture, depending upon the nature of the organisms isolated. Final report is at 5 days. |
| Specimen: |
Pus tissue, or other material properly obtained from an abscess, biopsy, aspirate, drainage, exudate, lesion, or wound. Specimens will also be cultured for aerobic growth. |
| Collect: |
Some anaerobes will be destroyed by contact with oxygen for only a few seconds. Overlying and adjacent areas of the wound/abscess must be carefully disinfected to eliminate contamination with normal aerobic flora. Ideally, pus or other fluid obtained by needle aspiration is transported to the lab as soon as possible in a capped syringe. Sampling of open lesions is enhanced by deep aspiration using a sterile catheter. Curettings of base of an open lesion are optimal. If irrigation is necessary, sterile normal saline may be used. |
| Volume: |
One anaerobic Culturette and two aerobic Culturettes or 0.5 mL pus or other fluid from aspirated site in syringe with needle removed and replaced with a cap. |
| Transport: |
Ambient. |
| Comment: |
Note suspected organism(s) on the test request form. Specimen which is not received in anaerobic transport swab or closed syringe or Refrigerate are unacceptable. Specimens from sites which have anaerobic bacteria as normal flora will be rejected (e.g., throat, feces, colostomy sites, rectal swabs, bronchial washes, cervical-vaginal mucosal swabs, sputum, skin and superficial wounds, voided or catheterized urine, and decubitus ulcer).
Unacceptable conditions: Nonsterile or leaking container, dry swab, dry material, syringes with needles attached. |
| Reference Range: |
No aerobic or anaerobic growth. |
| Additional Info: |
Culture of specimens from sites harboring endogenous anaerobic organisms or contaminated by endogenous organisms may be misleading with regard to etiology and appropriate therapy.
Gram stain performed. Organism identification is performed when clinically relavant organisms are suspected. Antimicrobial susceptibilities are performed on clinically relevant isolates when appropriate, following mentods (s) recommended by NCCLS. Call Laboratory for list of routine susceptibility panels. |
| LIS Code: |
ANACU |
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If you have questions about proper specimen collection, please call
NPHL Client Services at 402-559-2440 or toll free at 1-866-290-1406.
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