Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.


  • Bioterrorism Testing Capabilities at NPHL. Call 888-5588 for more information.


Sentinel Level Clinical Laboratory Guidelines

In coordination with the CDC and the Association of Public Health Laboratories (APHL), the ASM has developed protocols designed to assist Sentinel Level Clinical Microbiology Laboratories with information and techniques to rule out microorganisms that might be suspected as agents of bioterrorism, or to refer specimens to public health laboratories for confirmation. The Sentinel Level Clinical Microbiology Laboratory Guidelines offer standardized, practical methods to aid microbiologists in ruling out critical agents and referring specimens to public health laboratories (LRN Reference Laboratories) for confirmation.

Definition of LRN Sentinel Laboratory- Revised October 1, 2012

Laboratory Response Network (LRN) Information

BT Readiness Checklist for Sentinel Laboratories


Sentinel Laboratory procedures for other suspected agents of bioterrorism and emerging infectious diseases from ASM


  • Coxiella burnetii (Level-A (Sentinel) laboratories should not attempt to culture this organism.  Contact public health officials if Coxiella burnetii is suspected)
  • Clostridium botulinum (Laboratories should NOT attempt to culture this organism. Contact public health officials if botulinum susptected)


Select Agent Information


Biosafety in Microbiological and Biomedical Laboratories  (BMBL) 5th Edition

This edition of the BMBL continues to specifically describe combinations of microbiological practices, laboratory facilities, and safety equipment, and to recommend their use in four categories or biosafety levels of laboratory operation with selected agents infectious to humans.


Biosafety Level 2 (BSL2) Laboratory Biosafety Compliance Checklist

For Questions Contact:

Tony Sambol                                                 

Phone: 402-559-3032                                 

Pager: 402-888-5588                                   

Cell: 402-203-8498

Last Updated 03/21/2016